Journal: Cells
Article Title: PI 3-Kinase and the Histone Methyl-Transferase KMT2D Collaborate to Induce Arp2/3-Dependent Migration of Mammary Epithelial Cells
doi: 10.3390/cells13100876
Figure Lengend Snippet: PI 3-kinase activity is critical for collective cell migration. Confluent monolayers of parental, PIK3CA H1047R, KMT2D KO and double-mutant MCF10A cells are induced to migrate into the free space obtained by lifting a physical boundary. Displacement vectors within the monolayer are obtained using Particle Image Velocimetry (( A ), PIV). These vectors are analyzed over space and time for their position in terms of their magnitude (( B ), velocity) and directionality (( C ), order parameter, i.e., the cosine of the angle made by the displacement vector with respect to the vector oriented toward the free space). These parameters are represented as heat maps. There are three biological repeats with similar results, and four fields of view in each condition. Plots show the average of all measurements. Each pixel of the heatmaps averages all fields of view in all biological replicates over 42 µm and 40 min. These parameters are compared at a single time point or at a single distance from the edge using two-way ANOVA with Tukey’s test.
Article Snippet: Analysis of time-lapse images was performed using Particle Image Velocimetry (PIV) analysis with a MATLAB PIV script [ ].
Techniques: Activity Assay, Migration, Mutagenesis, Plasmid Preparation